Isolation and Characterization of a T Lymphocyte - Derived Differentiation Inducing Factor for the Myeloid Leukemic Cell
نویسندگان
چکیده
Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7.000-fold from HUT-i 02 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography. and preparative SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The final preparation is susceptible to protease treatment. has a molecular weight of 46,000. as determined by SDS-PAGE and approximately 55.000 by gel
منابع مشابه
Inducing Factor for the Myeloid Leukemic Cell Line Hl-60 Isolation and Characterization of a T Lymphocyte-derived Differentiation
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متن کاملIsolation and characterization of a T lymphocyte-derived differentiation inducing factor for the myeloid leukemic cell line HL-60.
Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7,000-fold from HUT-102 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography, and pre...
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